Anti-Human CD24 Recombinant Antibody
CAT#: TAB-113CL
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human CD24. Anti-CD24 antibodiess and adjuvant combinations thereof with chemotherapeutic agents or toxins, which can be used to inhibit growth of CD24-expressing cancer cells and prevent and treat cancer.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-Human CD24 Antibody (TAB-113CL) in ELISA
ELISA analysis of TAB-113CL was performed by coating with recombinant human CD24 (RPB143Hu01). Then blocked with BSA and incubated with Anti-Human CD24 Antibody (TAB-113CL) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human CD24 Antibody (TAB-113CL) in WB
Western blot analysis was performed by loading recombinant human CD24 (RPB143Hu01) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human CD24 Antibody (TAB-113CL) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Non-Reducing Antigen (0.05 μg)
Lane 2: Reducing Antigen (0.05 μg)
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Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45879/1529_A2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/1725_B2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_5_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (NTERA-2, RPTEC TERT1, RT4, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000272398-CD24
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Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Cancer
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
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Customer Review
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Perfect for Western Blot
I used the Anti-Human CD24 Recombinant Antibody for Western blotting, and it worked perfectly. The bands were sharp, and there was no background noise, even at a high concentration.
Efficient Cell Sorting
This antibody was perfect for sorting CD24 positive cells. The binding was specific, and the sorted cells maintained good viability. It's my go-to for cell sorting assays.
Cost-Effective and Reliable
Compared to other antibodies I've used, this one is both reliable and cost-effective. It gave consistent results across different experiments, making it an excellent investment for my research projects.
Q&As
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For Western blot applications, is there a recommended concentration?
A: For Western blot, the recommended antibody concentration is typically 1 µg/mL. However, optimization may be required based on the specific conditions of the experiment.
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Is it possible to use this antibody for Western blot?
A: Yes, the Anti-Human CD24 Recombinant Antibody can be used for Western blot.
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What role does CD24 play in the study of cancer?
A: CD24 is a marker often overexpressed in several cancers, including ovarian and breast cancer. It is associated with tumor progression and metastasis, making it a significant target in cancer research.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-113CL, RRID: AB_3111819)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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